DNA extraction from archived hematoxylin and eosin-stained tissue slides for downstream molecular analysis
نویسندگان
چکیده
منابع مشابه
Hematoxylin and Eosin Stained Tissue Using Color Brightfield Imaging with the CytationTM 5 to Image Fixed and Stained Tissue
Examination of chromogenically stained tissues has been the hallmark of clinical diagnostic pathology and cancer research for nearly a century. Fixed and stained tissue on microscope slides has been the routinely used method of data storage. Pathological samples to be viewed were routinely fixed and embedded in paraffin for long term storage. Thin slices were then made from the paraffin samples...
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DNA was isolated from 92 Giemsa-stained smears of lesions from suspected cases of cutaneous leishmaniasis and used for PCR-based diagnosis of Leishmania infection. Each smear had been examined under a light microscope at x 1,000 and scored for amastigote numbers. Although the smears had been stored for up to 4 years, all the microscopy-positive slides were also positive by PCR and four of the 1...
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UNLABELLED We propose a methodology for the design of features mimicking the visual cues used by pathologists when identifying tissues in hematoxylin and eosin (H&E)-stained samples. BACKGROUND H&E staining is the gold standard in clinical histology; it is cheap and universally used, producing a vast number of histopathological samples. While pathologists accurately and consistently identify ...
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Sections of formaldehyde-fixed, paraffin-embedded and routinely haematoxylin and eosin stained duodenal tissue of the domestic cat were inadvertently observed under blue excitation visible light of a fluorescence microscope, while undertaking histological studies on normal mammalian gastrointestinal tract. The sections revealed yellow fluorescing vesicular droplets in basally located cells with...
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Quantitative aspects of cytology and histology should be considered in diagnostic standardisation processes. The present paper summarises the cytological differences detected in 75 thyroid lesions using a computerized textural analysis. Cells stained with progressive hematoxylin and taken from paraffin blocks were overlaid with the extracted texture. This technique was based on the lineal detec...
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ژورنال
عنوان ژورنال: World Journal of Methodology
سال: 2019
ISSN: 2222-0682
DOI: 10.5662/wjm.v9.i3.32